Whole Blood Passage Time:

Clinical applications of whole-blood PCR with real-time instrumentation.

BACKGROUND As the genetic basis of many human diseases is being discovered, there is increasing need for the detection of single-nucleotide polymorphisms/mutations in medical laboratories. We describe an innovative approach that combines PCR amplification directly on whole blood and real-time detection PCR technology (WB-RTD PCR). METHODS We compared WB-RTD PCR with the method for extracted D...

Real-time cytotoxicity assays in human whole blood.

A live cell-based whole blood cytotoxicity assay (WCA) that allows access to temporal information of the overall cell cytotoxicity is developed with high-throughput cell positioning technology. The targeted tumor cell populations are first preprogrammed to immobilization into an array format, and labeled with green fluorescent cytosolic dyes. Following the cell array formation, antibody drugs a...

First passage time distribution in heterogeneity controlled kinetics: going beyond the mean first passage time

The first passage is a generic concept for quantifying when a random quantity such as the position of a diffusing molecule or the value of a stock crosses a preset threshold (target) for the first time. The last decade saw an enlightening series of new results focusing mostly on the so-called mean and global first passage time (MFPT and GFPT, respectively) of such processes. Here we push the un...

Whole blood filtration.

A whole blood oximeter.

During cardiac catheterization it is an advantage to have an immediate indication of the oxygen content of blood samples withdrawn from the heart and great vessels while the catheter is still in situ. This is especially important in the more complex congenital defects as the procedure can then be modified if necessary in the light of the blood oxygen estimations. The present paper describes a w...